微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pPLc24 PL-A |
|---|---|
| 商品货号： | TS198668 |
| Designations： | pPLc24 PL-A |
| Depositors： | Biogen, Inc., JF Haley, Biogen, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli M5219 Distribution host: Escherichia coli M5219 |
| Vector Information： | Size (kb): 3.1429998874664310 Vector: pPLc24 (plasmid) Promoters: Promoter lambda PL Construction: pBR322, MS2, lambda Marker(s):ampR Construct size (kb): 3.142999887466431 Features: marker(s): ampR promoter: lambda PL replicon: pMB1 enhancer: none |
| Applications： | expression vector |
| Comments： | The following unique restriction sites are found on this vector separated by (bp)(approx): BglI- 100- BamHI- 900- EcoRI- 600- XhoI- 300- SmaI- 250- HindIII- 1650. Restriction digests of the clone give the following sizes (kb): EcoRI--3.1; PvuI/BamHI--1.7, 1.4; BglI--3.1; PstI--3.1; HindIII--3.1. Plates equally well at 28C and 42C in E. coli K-12 deltaH1 hosts. Escherichia coli M5219 is Escherichia coli K-12 M72 lac(am) trp(am) rpsL lambda cI857 deltaH1 bio252. deltaH1 removes part of cro and all genes to the right of cro. bio252 removes all genes to the left of cIII. At 42C, N is expressed from the chromosome. Translation from the MS2 replicase is colinear with transcription from the PL promoter and thus is under PL control. Shows reduced plating efficiency in E. coli M5219 at 42C under antibiotic selection. This vector is used for expression of fused proteins with MS2 polymerase. ATG is in phase with GAT from the BamHI site, AAG from the HindIII site, TTA from the MstII site, CGC from the NruI site and GCT from the EspI site. The orientation of the PL promoter is clockwise with respect to the plasmid ori. This vector was constructed from pPLc28 (ATCC 31696) by inserting a 431 bp EcoRI/BamHI fragment coding for the ribosome binding site and the first 98 amino acids of the MS2 replicase (from pMS2-7) into pPLc28. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 28.0°C |
| References： | Fiers WC, Remaut ER. Vectors and methods for making such vectors and for expressing cloned genes. US Patent 4,874,702 dated Oct 17 1989 Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |