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| 产品名称： | pBBR1Tp |
|---|---|
| 商品货号： | TS198701 |
| Designations： | pBBR1Tp |
| Depositors： | DE Woods, D DeShazer |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 5.314 DESCRIPTION OF VECTOR: Intact vector size: 5.314 Type of vector: plasmid Cloning sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV PstI SmaI BamHI SpeI XbaI BstXI SacII SacI Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI BstXI SacII SacI Construction: pBBR1MCS, tmpR Host range: broad host range; Burkholderia pseudomallei; Burkholderia cepacia; Escherichia coli Features (with orientation and position when available): coding sequence: MOB, coding sequence: REP, ->, 1793-2455 MCS: SacI...KpnI, ->, 3220-3322 marker(s): tmpR, ->, 4387-4623 Vector: pBBR1Tp (plasmid) Construction: pBBR1MCS, tmpR Marker(s):tmpR Construct size (kb): 5.314 Features: marker(s): tmpR MCS: SacI...KpnI coding sequence: MOB coding sequence: REP |
| Applications： | cloning vector mobilizable vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--5.4; EcoRI--4.0, 0.64, 0.8; PvuII--2.4, 1.35, 1.1, 0.4 Plasmid is compatible with multiple replicons and can be transferred to other gram-negative bacteria by conjugation when the RK2 transfer functions are provided in trans. Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker. |
| Media： | ATCC® Medium 2031: LB + 1.5 mg/ml trimethoprim |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |