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| 产品名称： | Fgf8 cDNA1 mFgf8 |
|---|---|
| 商品货号： | TS198804 |
| Designations： | Fgf8 cDNA1 mFgf8 |
| GenBank Number： | Z46883 |
| Species： | Mus musculus, mouse |
| Depositors： | C Murphy, GR Martin |
| Applications： | To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.800000011920929 Gene product: fibroblast growth factor-8(androgen-induced growth factor) AIGF Alleles: b, b, s, s |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI/XhoI--3.0, 0.8; SmaI--3.8; XhoI--3.8; NcoI--3.65, 0.15; PstI--3.8. The insert contains the following restriction sites (approximate kb from the 5 end): BalI--0.14; NcoI--0.16; StyI--0.16. This insert is an alternate splice variant of Fgf8 that corresponds to bp 1-35 and 906-1038 of Z46883. To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Crossley PH, Martin GR. The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development 121: 439-451, 1995. PubMed: 7768185 |