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购买须知
| 产品名称： | pMTK116 |
|---|---|
| 商品货号： | TS199002 |
| Designations： | pMTK116 |
| Species： | Mus musculus, mouse |
| Depositors： | FH Ruddle |
| Vector： | Construct size (kb): 0.0 |
| Insert： | DNA: genomic Insert lengths(kb): 36.0 Tissue: liver Gene product: thymidine kinase 1, soluble Tk1 |
| Insert Size (kb)： | 36.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--12.0, 9.0, 8.0 doublet, 3.65; XhoI--41, 9.4; ClaI--35, 15; HindIII--23, 12, 5.5, 4.4; SalI/XhoI--9.0, 8.0, 7.5, 6.2, 5.5, 4.9, 3.35. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Insert includes the entire coding sequence as well as 5 and 3 flanking sequences on a 22 kb HpaI fragment. Promoter is either within or flanking the 5 end of the ApaI/XhoI fragment. Gene spans about 11 kb. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Lin PF, et al. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. 5: 3149-3156, 1985. PubMed: 3018504 Fiorentino L, et al. Assignment of the interleukin-2 locus to mouse chromosome 3. Genomics 5: 651-653, 1989. PubMed: 2613246 |