| 产品名称: | Ac2 |
|---|---|
| 商品货号: | TS199025 |
| Designations: | Ac2 |
| GenBank Number: | J04210 |
| Species: | Homo sapiens, human |
| Depositors: | K Tryggvason |
| Vector: | Construct size (kb): 6.699999809265137 |
| Insert: | DNA: cDNA Insert lengths(kb): 4.0 Tissue: placenta Gene product: collagen, type IV, alpha 2 COL4A2 |
| Insert Size (kb): | 4.0 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | The insert contains the following restriction sites (in bp from the 5 end of the complete cDNA): BamHI--2505, 2540, 3864, 3908; SphI--3083. Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.7; BamHI--4.4, 1.3, 1.0; PstI--6.7; XbaI--6.7. On its 3 end, Oc7 (ATCC 65038, 65039) overlaps Ac2 (TS199025, 65041) by about 750 base pairs. On its 3 end, Ac2 overlaps HC-47 (ATCC 65042, 65043) by about 2400 base pairs. The insert encodes most of the collagenous domain and part of the non-collagenous domain. |
| References: | Hostikka SL, Tryggvason K. The complete primary structure of the alpha2 chain of human type IV collagen and comparison with the alpha1(IV) chain. J. Biol. Chem. 263: 19488-19493, 1988. PubMed: 3198637 |