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| 产品名称： | pTK-HSV-BP2 plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS199064 |
| Designations： | pTK-HSV-BP2 plasmid in Escherichia coli |
| GenBank Number： | U02031 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Applications： | in suitable host, produces protein sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2 |
| Vector： | Name of vector: pTK-HSV Intact vector size: 5.8 Type of vector: phagemid Vector end: BspDI Vector end: XbaI Cloning sites: BspDI XbaI Construction: pcDNA3 Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1 marker(s): neoR, ← replicon: f1, ← terminator: bGH polyadenylation restriction site: XbaI restriction site: BspDI epitope tag: HSV promoter: HSV TK, ← marker(s): ampR, → |
| Insert： | Insert size (kb): 4.2 Gene: sterol regulatory element binding transcription factor 2, SREBP2 DNA: cDNA Source: human Genbank accession : U02031 Nucleotides: Nucleotides 1-4093 of the insert correspond to nucleotides 156-4249 of U02031 |
| Insert Size (kb)： | 4.2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): BspDI/XbaI--5.8, 4.2; EcoRI--6.8, 3.0; HindIII--9.0; 0.8. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--1.08; HindIII--1.12; BglII--1.70; EcoRI--2.45 This construct allows the exogenously expressed SREBF2 to be cleaved in a sterol-regulated fashion after transfection into 293 cells (ATCC CRL-1573). The amino terminus was truncated at amino acid 14 and fused to the HSV tag of the vector. |
| References： | Hua X, et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. J. Biol. Chem. 270: 29422-29427, 1995. PubMed: 7493979 Joseph L Goldstein, personal communication |