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| 产品名称： | pALCA1SIFN |
|---|---|
| 商品货号： | TS199113 |
| Designations： | pALCA1SIFN |
| Species： | Homo sapiens, human |
| Depositors： | Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| Applications： | in another host, produces protein interferon, alpha 2 |
| Vector： | Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pALCA1S Intact vector size: 4.500 Type of vector: plasmid Vector end: BamHI Vector end: EcoRI Cloning sites: EcoRI, BamHI Polylinker sites: Other unique sites: HindIII Construction: pDG6 (ATCCC 53169) Host range: Escherichia coli; filamentous fungi Features (with orientation and position when available): promoter: alcA, -> signal peptide coding region: synthetic, -> MCS: XbaI...EcoRI marker(s): ampR replicon: pMB1 marker(s): ARGB Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNA2 Genomic copy number: unique Gene name: interferon, alpha 2 Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 p22 Type of DNA: cDNA Insert 5 end: BglII Insert 3 end: EcoRI Insert size (kb): 0.9 Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: interferon, alpha 2 IFNA2 Target Gene: interferon, alpha 2 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--4.5, 0.9; HindIII--5.4, EcoRI--5.4. This plasmid may be difficult to isolate and digest. If this problem occurs, increase the final concentration of ampicillin in the growth mediun to 1 mg/ml. Transformants secrete protein, inducible by threonine and repressible by glucose. |
| References： | Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |