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微信小章| 产品名称： | p627 |
|---|---|
| 商品货号： | TS199115 |
| Designations： | p627 |
| GenBank Number： | X03484 |
| Species： | Homo sapiens, human |
| Depositors： | UA Rapp |
| Applications： | produces protein murine leukemia viral (v-raf-1) oncogene homolog 1 Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1 |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.900000095367432 Tissue: fetal liver Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) RAF1 Alleles: B, M, B1, A1, A2, B2 Target Gene: murine leukemia viral (v-raf-1) oncogene homolog 1 |
| Insert Size (kb)： | 2.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: Frozen |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.9, 2.7; HindIII--5.25, 0.55; BglII--5.7; XbaI--3.5, 2.2. RAF1 shows linkage to Von Hippel-Lindau disease (VHL) with a LOD score of 4.38 with theta = 0.11. Haplotype frequencies for the TaqI and BglI polymorphisms suggest only slight linkage disequilibrium between the two sites. Significant linkage has been demonstrated to von Hippel-Lindau disease (theta = 0.0, z = 2.6). Insert contains PvuII (2), HindII, SalI (2), StuI (3), SphI, BglII, EcoRV, and Xba sites. |
| References： | Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734 Bonner TI, et al. Structure and biological activity of human homologs of the raf/mil oncogene. Mol. Cell. Biol. 5: 1400-1407, 1985. PubMed: 2993863 Elliott RW, et al. Localization of the rhodopsin gene to the distal half of mouse chromosome 6. Genomics 6: 635-644, 1990. PubMed: 2341153 Vance JM, et al. Confirmation of linkage in von Hippel-Lindau disease. Genomics 6: 565-567, 1990. PubMed: 2328994 Bonner TI, et al. The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. Nucleic Acids Res. 14: 1009-1015, 1986. PubMed: 3003687 Seizinger BR, et al. Von-Hippel Lindau disease maps to the region of chromosome 3 associated with renal cell carcinoma. Nature 332: 268-269, 1988. PubMed: 2894613 Ulf A Rapp, personal communication |