| 产品名称: | int-2d |
|---|---|
| 商品货号: | TS199157 |
| Designations: | int-2d |
| GenBank Number: | Y00848 |
| Species: | Mus musculus, mouse |
| Depositors: | C Dickson, G Peters |
| Vector: | Construct size (kb): 5.099999904632568 |
| Insert: | DNA: genomic Insert lengths(kb): 2.099999904632568 Gene product: fibroblast growth factor-3( V-INT2 murine mammary tumor virus integration site oncogene homolog, murine mammary tumor virus integration site (v-int-2) oncogene homolog, murine mammary tumor virus integration site 2, mouse, included) Int2 Alleles: a, b |
| Insert Size (kb): | 2.100 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): SacI--3.0 (doublet), 2.1; EcoRI/HindIII--3.0 (doublet), 2.1; XbaI--5.1, 3.0; PstI--3.0, 3.3, 1.8; BamHI--4.7, 3.0, 0.4. The insert contains the following restriction sites (with nucleotide positions according to the published sequence): KpnI--2527, 3804; BamHI--2749; NotI--2922. The plasmid contains two copies of the pSP65 sequence as well as the 2.1 kb insert. The insert extends from nt 2339 to 4459 of the published sequence and includes exon 2 and flanking intronic regions. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Dixon M, et al. Detection and characterization of the fibroblast growth factor-related oncoprotein INT-2. Mol. Cell. Biol. 9: 4896-4902, 1989. PubMed: 2557543 Peters G, et al. Mouse mammary tumor virus integration regions int-1 and int-2 map on different mouse chromosomes. Mol. Cell. Biol. 4: 375-378, 1984. PubMed: 6321961 Dickson C, et al. Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2. Cell 37: 529-536, 1984. PubMed: 6327073 Moore R, et al. Sequence, topography, and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammry tumor virus. EMBO J. 5: 919-924, 1986. PubMed: 3013624 Clive Dickson, personal communication |