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| 产品名称： | 6c |
|---|---|
| 商品货号： | TS199221 |
| Designations： | 6c |
| GenBank Number： | X14480 |
| Species： | Mus musculus, mouse |
| Depositors： | M Chu |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.5 Tissue: Engelbreth-Holm-Swarm (EHS) tumor Gene product: nidogen Nid Alleles: B, S |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--4.5; HindIII--4.5; PvuI--3.5, 1.0. Restriction digests of the clone give the following sizes (kb): PvuII--2.8, 0.9, 0.8. Contains a PstI site (nt 2627 of the published sequence) which is approximately 600 bp from the 5 EcoRI site. Corresponds to sequences beginning about 1.5 kb upstream of the EcoRI site at nt 3525 of the published sequence, and encodes most of the rod-like domain and part of the C-terminal globular domain. Detects mRNA of 6.0 and 4.0 kb (weak) in the mouse and 6.0 kb in the human. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Jenkins NA, et al. Nidogen/entactin (Nid) maps to the proximal end of mouse chromosome 13 linked to beige (bg) and identifies a new region of homology between mouse and human chromosomes. Genomics 9: 401-403, 1991. PubMed: 1672300 Mann K, et al. Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. EMBO J. 8: 65-72, 1989. PubMed: 2496973 Mon-Li Chu, personal communication |