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| 产品名称： | p3IT |
|---|---|
| 商品货号： | TS199224 |
| Designations： | p3IT |
| GenBank Number： | X52634 |
| Species： | Mus musculus, mouse |
| Depositors： | ML Lane |
| Vector： | Construct size (kb): 8.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 4.699999809265137 Tissue: NIH 3T3 cells transformed by DNA from S49 lymphoma Gene product: T lymphoma oncogene Tlm |
| Insert Size (kb)： | 4.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The EcoRI/BamHI fragment contains the following restriction sites (in nucleotides relative to the published sequence): XbaI--208, PstI--348, 455, 1038, 1103; NdeI--650, 823; PvuII--1639, EcoRI--2221. The coding sequences are contained in a EcoRV/BamHI fragment of about 3.25 kb which has been sequenced. Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--5.0, 4.1; HindIII--9.2; BamHI--9.2; PstI--5.8, 1.15; 1.07; 0.64; EcoRI--5.2, 4.1; SalI--9.2. p3IT retains transforming activity which is inactivated by cleavage with EcoRI but not BamHI, HindIII, or EcoRV. Introns within the clone hybridize to highly repetitive mouse sequences. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Lane MA, Tobin MB. Genomic sequence of the mouse oncogene tlm. Nucleic Acids Res. 18: 3410, 1990. PubMed: 2356136 Lane MA, et al. Isolation and characterization of a stage-specific transforming gene, Tlym-I, from T-cell lymphomas. Proc. Natl. Acad. Sci. USA 81: 2227-2231, 1984. PubMed: 6326114 |