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| 产品名称： | pBLCAT5 |
|---|---|
| 商品货号： | TS199310 |
| Designations： | pBLCAT5 |
| Depositors： | M Boshart |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4000000953674320 Vector: pBLCAT5 (plasmid) Promoters: Promoter HSV TK Construction: pBLCAT2 Marker(s):ampR Construct size (kb): 4.400000095367432 Features: marker(s): ampR promoter: HSV TK replicon: pMB1 terminator: SV40 polyadenylation |
| Applications： | reporter construct vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.6, 0.4; PstI--4.2, 0.2; PvuII--2.5, 1.8; BglI/XhoI--2.8, 1.55; ClaI/XhoI--4.4. The vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.03; BglI--3.23; ClaI--1.84; EcoRI--0.06, 0.46, 1.86; HindIII--0.0; NcoI--0.76; PstI--0.01, 0.16; PvuI--3.48; PvuII--0.35, 2.04; SacI--1.86; XhoI--0.20. Reporter plasmid for analysis of enhancer sequences in vertebrate cells, using the herpes simplex virus (HSV) thymidine kinase promoter to drive expression of the CAT reporter gene. Constructed from pBLCAT2 (ATCC 37527) by deletion of a 467 bp fragment immediately upstream of the 5 polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/BamHI - HSV tk promoter - cat - SV40 small T splice signal - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Boshart M, et al. Reporter constructs with low background activity utilizing the cat gene. Gene 110: 129-130, 1992. PubMed: 1544570 |
| Shipped： | freeze-dried |