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购买须知
| 产品名称： | MEF (CF-1) IRR |
|---|---|
| 商品货号： | TS199343 |
| Organism： | Mus musculus, mouse |
| Tissue： | Embryo |
| Cell Type： | Fibroblast |
| Product Format： | frozen |
| Morphology： | Fibroblast |
| Culture Properties： | Adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 14 days gestation embryo |
| Gender： | male and female mixed |
| Strain： | CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms |
| Applications： | Irradiated cells for use as feeder layer |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male and female mixed |
| Comments： | These cells have been growth-arrested by irradiation with 10,000 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended). |
| Subculturing： | Medium Renewal: Twice a week or when pH decreases |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | ATCC |
| Year of Origin： | 2004 |
| References： | Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003. |