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| 产品名称： | pJA70 |
|---|---|
| 商品货号： | TS199515 |
| Designations： | pJA70 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.0780000686645510 DESCRIPTION OF VECTOR: Intact vector size: 5.078 Type of vector: plasmid Cloning sites: SacI SacII NotI XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI ApaI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI Construction: pUC, Tn903 Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): centromere: CEN6 insert detection: lacZ marker(s): kanR, HIS3 promoter: lac replicon: pMB1, ARSH4 Vector: pJA70 (plasmid) Promoters: Promoter lac Construction: pUC, Tn903 Marker(s):kanR,HIS3 Construct size (kb): 5.078000068664551 Features: insert detection: lacZ marker(s): kanR, HIS3 promoter: lac replicon: pMB1, ARSH4 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector vector for other uses vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.2, 1.8, 1.2; HindIII--3.1, 1.9, 0.2; KpnI--2.9, 2.1; EcoRI--5.1. YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffrons laboratory. Permits visual detection of recombinants by the lacZalpha complementation system. The order of the major features in this plasmid is: lacZ/MCS - CEN6 - ARS H4 - HIS3 - kanR - pMB1 ori. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Related Products： | component of:ATCC 77261 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |