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| 产品名称： | phPMP22-1 |
|---|---|
| 商品货号： | TS199583 |
| Designations： | phPMP22-1 |
| GenBank Number： | M94048 |
| Species： | Homo sapiens, human |
| Depositors： | PI Patel |
| Vector： | Construct size (kb): 3.674000024795532 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCR1000 Intact vector size: 3.000 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): kanR promoter: lac, T7 replicon: ColE1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PMP22 Genomic copy number: unique Gene name: peripheral myelin protein 22 Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 p12-p11.2 Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: EcoRI Insert size (kb): 0.674 Cross references: DNA Seq. Acc.: M94048 Insert lengths(kb): 0.6740000247955322 Gene product: peripheral myelin protein 22 PMP22 |
| Insert Size (kb)： | 0.674 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.75; BglI--3.2, 0.55; EcoRI--3.7; PstI--2.1, 1.6; SacI--3.7. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.09; BglI--0.32. The gene appears to be located entirely within the Charcot-Marie-Tooth disease (CMT1A) duplication. Detects genomic restriction fragments of the following sizes (kb): human, HindIII--7.4, 2.5; mouse, HindIII--5.1, 2.1; hamster, HindIII--7.5, 2.8; human, EcoRI--11, 7.9. Corresponds to nt 147-820 of the published sequence, and contains the complete coding region (nt 50-529 of the published sequence). Detects transcripts of 1.8, 1.3, and 0.8 kb. |
| References： | Patel PI, et al. The gene for the peripheral myelin protein PMP-22 is a candidate for Charcot-Marie-Tooth disease type 1A. Nat. Genet. 1: 159-165, 1992. PubMed: 1303228 |