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| 产品名称： | Canine distemper virus |
|---|---|
| 商品货号： | TS199760 |
| Classification： | Paramyxoviridae, Morbillivirus |
| Agent： | Canine distemper virus |
| Strain： | Snyder Hill (cell culture adapted) |
| Applications： | It is used primarily as a challenge strain to test dogs for immunity to the disease.
ATCC VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950.
This virus produces neurological signs with regularity only when inoculated intracerebrally.
It does not produce hemagglutinin or hemolysin.
Respiratory research |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Neurological signs are produced with regularity only when inoculated intracerebrally. This strain does not produce hemagglutinin or hemolysin. It is used primarily as a challenge strain to test dogs for immunity to this disease. Cross-immunity tests in dogs have shown it to be similar to other classical strains of CDV. |
| Effect on Host： | CPE, vacuolization and rounding of cells, sloughing |
| Recommended Host： |
Vero cells (ATCC® CCL-81™)
|
| Growth Conditions： | Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 90% confluent (not 100% confluent). Incubation: 5 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended |
| Name of Depositor： | LE Carmichael, ATCC |
| Special Collection： | ATCC |
| Source： | ATCC® VR-1587™ was derived at ATCC from ATCC®xa0VR-526™ by adaptation to growth in Vero cells (ATCC® CCL-81™). ATCC® VR-526™, deposited by L.E. Carmichael, was isolated from brain of dog with clinical distemper, September, 1950. Because it was propagated in animal tissue ATCC will no longer offer that product. |
| References： | Gillespie JH. . Am. J. Vet. Res. 17: 103, 1956. PubMed: 13259024 |