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微信小章| 产品名称： | Poterioochromonas malhamensis (Pringsheim) Peterfi |
|---|---|
| 商品货号： | TS199800 |
| Deposited As： | Ochromonas malhamensis Pringsheim |
| Application： | assay of vitamin B12 cobalamin, cobalamins |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | test tube |
| Type Strain： | no |
| Comments： | characterization of mitochondrial genome comparison of methods of vitamin analysis water-soluble vitamins in cells and spent culture supernatants photosynthetic |
| Medium： | ATCC® Medium 891: Ochromonas medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium. |
| Subcultivation： | Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium. |
| Name of Depositor： | SH Hunter |
| Chain of Custody： | ATCC < |
| References： | Coleman AW, et al. Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica. J. Protozool. 38: 129-135, 1991. Analytical microbiology. New York: Academic Press; 1963. Analytical microbiology. New York: Academic Press; 1963. Voight MN, et al. Comparison of Protozoan and conventional methods of Vitamin analysis. J. Food Sci. 44: 729-734, 1979. Voigt MN, et al. Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci. 44: 724, 1979. Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981. Ghione M, DellOrto P. Human polypeptidic hormone-like substances in microorganisms. Microbiologica 6: 315-326, 1983. PubMed: 6319976 Ford JE. The microbiological assay of vitamin B12; the specificity of the requirement of Ochromonas malhamensis for cyanocobalamin. Br. J. Nutr. 7: 299-306, 1953. |