Construct size (kb): 4.699999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HSPCA
Other gene symbols: HSPC1
Gene name: heat shock 90kD protein 1, alpha
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M78050; EST: EST01637; Primer: M13 Reverse;
THC id: THC123959

Insert lengths(kb): 1.700000047683716

Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)

Gene product: heat shock 90kD protein 1, alpha HSPC1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.5; PstI--3.2, 1.2, 0.2; BamHI--3.4, 1.1.

EST01637 matches GenBank accession HUMHSP90 with 99.62% identity over the sequence range: 1-266.

Sequenced from the M13 reverse primer.