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| 产品名称： | pUC-h27OH |
|---|---|
| 商品货号： | TS199843 |
| Designations： | pUC-h27OH |
| GenBank Number： | M62401 |
| Species： | Homo sapiens, human |
| Depositors： | DW Russell |
| Applications： | contains sequence cytochrome P-450, sterol 27-hydroxylase cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase |
| Vector： | Construct size (kb): 4.579999923706055 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.884999990463257 Tissue: human prostate Gene product: cytochrome P-450, sterol 27-hydroxylase( cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase) h27OH Target Gene: cytochrome P-450, sterol 27-hydroxylase |
| Insert Size (kb)： | 1.885 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; HindIII--3.0, 1.6; XbaI--4.6; BamHI--3.0, 1.6; PstI--2.9, 0.8, 0.7, 0.2. DNA sequence analysis of hybridization-positive clones predicted a human sterol 27-hydroxylase consisting of a 33-amino-acid mitochondrial signal sequence followed by a mature protein of 498 amino acids. |
| References： | Cali JJ, Russell DW. Characterization of human sterol 27-hydroxylase. A mitochondrial cttochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis. J. Biol. Chem. 266: 7774-7778, 1991. PubMed: 1708392 |