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微信小章| 产品名称： | Trypanosoma cruzi Chagas |
|---|---|
| 商品货号： | TS199845 |
| Strain Designations： | M/HOM/AR/74/CA-I CL72 |
| Application： | Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clone 72 derived from strain CA-I, originally isolated from a human male with chronic myocarditis, San Luis Province, Argentina, 1974, cloned by J. Dvorak, 1980 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | characterization flow cytometric analysis growth kinetics of clones in liquid medium |
| Medium： | ATCC® Medium 1029: LIT medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic |
| Cryopreservation： | 1.xa0xa0 Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min. 2. xa0 Adjust concentration of cells to 2 x 107/ml in fresh medium. 3. xa0 While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use. 4. xa0 Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min. 5. xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0xa0 Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 7.xa0xa0 Store in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0xa0 To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed. 9.xa0xa0 Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 ml ATCC medium 1029.xa0 10.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Incubate at 25°C with the cap screwed on tightly. 11.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Maintain as described above.xa0 |
| Name of Depositor： | JA Dvorak |
| Special Collection： | NCRR Contract |
| Year of Origin： | 1974 |
| References： | Dvorak JA, et al. Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence. J. Protozool. 29: 430-437, 1982. PubMed: 6182288 Engel JC, et al. Trypanosoma cruzi: biological characterization of 19 clones derived from two chronic chagasic patients. I. Growth kinetics in liquid medium. J. Protozool. 29: 555-560, 1982. PubMed: 6816924 Nozaki T, et al. Cellular and molecular biological analyses of nifurtimox resistance in Trypanosoma cruzi. Am. J. Trop. Med. Hyg. 55: 111-117, 1996. PubMed: 8702014 Engel JC, et al. Trypanosoma cruzi: biological characterization of clones derived from chronic chagasic patients. II. Quantitative analysis of the intracellular cycle. J. Protozool. 32: 80-83, 1985. PubMed: 3921699 Nozaki T, Dvorak JA. Intraspecific diversity in the response of Trypanosoma cruzi to environmental stress. J. Parasitol. 79: 451-454, 1993. PubMed: 8501607 Doyle PS, et al. Trypanosoma cruzi: quantification and analysis of the infectivity of cloned stocks. J. Protozool. 31: 280-283, 1984. PubMed: 6381718 Engel JC, et al. Isolate-dependent differences in the oxidative metabolism of Trypanosoma cruzi epimastigotes. Mol. Biochem. Parasitol. 39: 69-76, 1990. PubMed: 2406595 |