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| 产品名称： | p2A-E3 pCD-2A-E3 |
|---|---|
| 商品货号： | TS199855 |
| Designations： | p2A-E3 pCD-2A-E3 |
| Species： | Mus musculus, mouse |
| Depositors： | T Yokota |
| Applications： | Induces significant Ia activity and enhances production of IgE and IgG1 by LPS-stimulated B cells (BSF-1 activity). |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.5899999737739563 Tissue: T-lymphocyte Cl.Ly1+2-/9 cell line Gene product: interleukin 4 Il4 |
| Insert Size (kb)： | 0.590 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Contains internal ClaI (1), PstI (1), ScaI (1), and XmnI (1) sites. Restriction digests of the clone give the following sizes (kb): BamHI--4.3, 0.76 (doublet); PstI--3.5, 1.3, 0.4; ScaI--3.6, 1.4, 0.76; AvaI/ScaI--2.4, 1.4, 1.2; ClaI--4.2, 0.92, 0.76. Has TCGF (T-cell growth factor) activity on HT-2 cells and MCGF (mast cell growth factor) activity on MC/9 cells. Induces significant Ia activity and enhances production of IgE and IgG1 by LPS-stimulated B cells (BSF-1 activity). The BamHI fragment of pCD-2A-E3 (approx. 0.76 kb) containing interleukin 4-coding sequences plus some pCD vector sequences (total insert size is approximately 750 bp) was cloned into the BamHI site of pBR322.There are two copies of the insert. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412 |