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微信小章| 产品名称： | asb4/134 |
|---|---|
| 商品货号： | TS199936 |
| Designations： | asb4/134 |
| GenBank Number： | X15617 |
| Species： | Mus musculus, mouse |
| Depositors： | AJ Ouellette |
| Applications： | This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library. |
| Vector： | Construct size (kb): 4.679999828338623 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3149999976158142 Tissue: adult small intestine Gene product: defensin-related cryptdin peptide Defcr Alleles: b, a, c |
| Insert Size (kb)： | 0.315 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites separated by (bp): (PstI)- 46- AvaII- 54- HinfI- 12- BglII- 2- Sau3A, XhoII- 15- Sau3A- 45- PstI- 18- RsaI- 54- RsaI- 19- BanI- 48- (PstI). Restriction digests of the clone give the following sizes (kb): PstI--4.5, 0.16 (doublet); EcoRI--4.8; HindIII--4.8; BamHI--4.8; AvaI--4.8; PvuI/NdeI--2.9, 1.8. The cryptdin mRNA (450 - 500 nt in length) is abundant in Paneth cells and can be detected in the testis and the brain. The gene maps to the proximal region of chromosome 8, within 1.28-21.27 cM of the Xmv-26 locus. Contains a open reading frame encoding a highly polar protein of 70 aa with all 6 cysteine residues in the COOH-terminal half. There are about 100 untranslated nucleotides 3, including poly(A). The insert lacks 115 bp of 5-terminal sequence of the mRNA (which has a total size of 0.45 kb). This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Ouellette AJ, et al. Localization of the cryptdin locus on mouse chromosome 8. Genomics 5: 233-239, 1989. PubMed: 2571573 Ouellette AJ, et al. Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium. J. Cell Biol. 108: 1687-1695, 1989. PubMed: 2715173 Ouellette AJ, Lualdi JC. A novel mouse gene family coding for cationic, cysteine-rich peptides: regulation in small intestine and cells of myeloid origin. J. Biol. Chem. 265: 9831-9837, 1990. PubMed: 2351676 Andre J Ouellette, personal communication |