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| 产品名称： | p424 CYC1 |
|---|---|
| 商品货号： | TS199966 |
| Designations： | p424 CYC1 |
| Depositors： | M Funk |
| Other IDs： | none : 87381 Sequence from depositor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1339998245239260 Vector: p424 CYC1 (plasmid) Promoters: Promoter for expression CYC1 Construction: pRS424 Marker(s):TRP1,ampR Construct size (kb): 6.133999824523926 Features: marker(s): TRP1 marker(s): ampR promoter for expression: CYC1 replicon: 2 micron terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; SacI--6.2; SacI/XbaI--3.6, 1.8, 1.1, 0.3; XbaI--3.5, 1.6, 0.94. Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | freeze-dried |