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| 产品名称： | pSNATCH |
|---|---|
| 商品货号： | TS199995 |
| Designations： | pSNATCH |
| Depositors： | C Hovens |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0819997787475590 Vector: pSNATCH (phagemid) Promoters: Promoter CMV Construction: pSCTEV, pSuperCATCH-NLS Marker(s):ampR Construct size (kb): 5.081999778747559 Features: marker(s): ampR other: VP16 activation domain promoter: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: M13 replicon: SV40 ori replicon: pMB1 replicon: polyoma MCS: SfuI...XbaI signal peptide coding region: SV40 NLS terminator: SV40 epitope tag: FLAG |
| Applications： | encodes removable tag for protein isolation expression vector vector for assessing nuclear localization signals vector permitting construction of fusion proteins vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--5.1; PstI--3.7, 1.4. The vector was constructed as follows: a 120bp VP16 fragment from pSCTEV Gal4p/VP16 was ligated to adaptors and cloned into BamHI cut pSuperCATCH-NLS. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205 |
| Related Products： | component of:ATCC 87677 |
| Shipped： | freeze-dried |