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微信小章| 产品名称： | PI-11 |
|---|---|
| 商品货号： | TS200006 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | It reacts with a single protein in Western blots of A-431 cells (ATCC CRL-1555) corresponding to purified 130 kDa myocardial calpastatin. The antibody is specific for human calpastatin. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting calpastatin. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with calpastatin isolated from human erythrocytes. The antibody is specific for human calpastatin. It reacts with a single protein in Western blots of A-431 cells (ATCC CRL-1555) corresponding to purified 130 kDa myocardial calpastatin. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human calpastatin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human calpastatin |
| Comments： | Animals were immunized with calpastatin isolated from human erythrocytes. The antibody is specific for human calpastatin. It reacts with a single protein in Western blots of A-431 cells (ATCC CRL-1555) corresponding to purified 130 kDa myocardial calpastatin. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting calpastatin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. An inoculum of 5 x 103 to 3 x 104 viable cells/mL is recommended. Maintain cell density between 1 x 104 and 1 x 105 viable cells/mL. Do not exceed 5 x 105 cells/mL. Medium Renewal:xa0 Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 kappa , IgG1; kappa light chain |
| Population Doubling Time： | 15 hours |
| Name of Depositor： | R Lane, RL Mellgren |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1990 |
| References： | Lane RD, Mellgren RL. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203: 5-16, 1992. PubMed: 1426051 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |