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| 产品名称： | pBR327 |
|---|---|
| 商品货号： | TS200021 |
| Designations： | pBR327 |
| Depositors： | EM Lederberg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.2730000019073490 Vector: pBR327 (plasmid) Marker(s):ampR,tetR Construct size (kb): 3.273000001907349 Features: marker(s): ampR, tetR replicon: pMB1 |
| Comments： | A general purpose plasmid vector. Derived from pBR322 by deleting sequences between nt 1430 (AvaI) and 2519, thus removing the bom or Mob site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Recomb. DNA Tech. Bull. 2: 1980.. Twigg AJ, Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature 283: 216-218, 1980. PubMed: 7350544 Covarrubias L, et al. Construction and characaterization of new cloning vehicles V. mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13: 25-35, 1981. PubMed: 6263753 Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735 |
| Shipped： | freeze-dried |