微信小陈
微信小章| 产品名称： | Feline immunodeficiency virus |
|---|---|
| 商品货号： | TS200068 |
| Classification： | Retroviridae, Lentivirus |
| Deposited As： | Feline Immunodeficiency Virus |
| Agent： | Feline immunodeficiency virus |
| Strain： | NCSU1 |
| Applications： | Isolate of Feline Immunodeficiency Virus (FIV) which is highly infectious in vivo and produces a rapid inversion of the CD4+/CD8+ receptor ratio in infected cats.
Pretreat host cells with DEAE-dextran (25 µg/mL). |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | This item is highly infectious in vivo and produces a rapid inversion of the CD4+/CD8+ receptor ratio in infected cats. Viral reverse transcriptase is Mg-dependent. |
| Effect on Host： | CPE, syncytia and cell lysis |
| Recommended Host： | MYA-1 cells (ATCC® CRL-2417™) |
| Growth Conditions： |
Temperature: 37°C Recommendations for Infection: For best results, pre-treat cells with 25 µg/mL DEAE-Dextran (MP Biomedicals 02195133) for 30 minutes at 23°C. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Add virus dilution to host cells and rock or gently vortex every 15-20 minutes for the first 1-2 hours of incubation at 37°C. Re-suspend cells at an appropriate density. Incubate at 37°C in a humidified 5% CO2 atmosphere. Incubation:xa012-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the culture. |
| Name of Depositor： | WAF Tompkins, North Carolina State University |
| U.S. Patent： | |
| U.S. Patent Number： | |
| References： | Tompkins WAF, Tompkins MB. Feline immunodeficiency virus isolate NCSU.sub.1Lb. US Pat. 5,413,927 dated May 9, 1995. |