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| 产品名称： | LA07NS01 |
|---|---|
| 商品货号： | TS200133 |
| Designations： | LA07NS01 |
| GenBank Definition： | H.sapiens CD36 gene exon 1. H.sapiens CD36 gene exon 2. |
| GenBank Number： | Z32752 Z32753 |
| Species： | Homo sapiens, human |
| Depositors： | LL Deaven |
| Origin： | hamster/human MR3.31 6TG6 hybrid cell line |
| Applications： | This is preserved in a solution containing 10% glycerol and 1.4 M CsCl. Of 496 probes analyzed after screening for human/hamster repeats, 40% were hamster specific, 39 contained moderately repetitive elements, and 258 (52%) were human-specific single-copy probes. |
| Vector： | Tissue: hamster/human MR3.31 6TG6 hybrid cell line Chromosome: 7 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 9.199999809265137 Coverage: Clones for "complete" coverage: 26000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | This is preserved in a solution containing 10% glycerol and 1.4 M CsCl. The CsCl should be diluted to less than 40 mM before infection. About 26% hamster impurity by in situ hybridization of sorted chromosomes; about 48% hamster impurity by plaque hybridization. Hamster impurity is 20-30%. Of 496 probes analyzed after screening for human/hamster repeats, 40% were hamster specific, 39 contained moderately repetitive elements, and 258 (52%) were human-specific single-copy probes. The size range of these was 0.5 - 6.5 kb. Of 55 unique sequence clones, 53 map to chromosome 7. Of 19 unique sequence clones, 18 map to chromosome 7. This is the second amplification of this library. |
| References： | Rommens JM, et al. Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene. Am. J. Hum. Genet. 43: 645-663, 1988. PubMed: 2903665 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 |