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微信小章| 产品名称： | EN9F10 |
|---|---|
| 商品货号： | TS200174 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody detects ergopeptine and lysergic acid derivatives. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid) |
| Comments： | The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody detects ergopeptine and lysergic acid derivatives. The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension atxa0 2xa0 to 5xa0 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.xa0 Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG2b |
| Name of Depositor： | RA Shelby |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Shelby RA, Kelley VC. Detection of ergot alkaloids in tall fescue by competitive immunoassay with a monoclonal antibody. Food Agric. Immunol. 3: 169-177, 1991. Shelby RA, Kelley VC. Detection of ergot alkaloids from Clavicops species in agricultural products by competitive ELISA using a monoclonal antibody. J. Agric. Food Chem. 40: 1090-1092, 1992. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |