微信小陈
微信小章| 产品名称： | Ca Ski |
|---|---|
| 商品货号： | TS200225 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix; Derived fom metastatic site: small intestine |
| Cell Type： | epithelial cell (CRM) |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma virus Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | epidermoid carcinoma |
| Age： | 40 years adult |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0 |
| Intended Use： | Certified Reference Material produced under an ISO 17034 accredited process.
xa0xa0xa0 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from cells from a metastasis in the small bowel mesentery. |
| Clinical Data： | female Caucasian 40 years adult |
| Genes Expressed： | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
| Cellular Products： | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
| Comments： | Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO 17034 accredited process.
The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. NOTE: Virology taxonomy has discontinued use of the family name "Papovaviridae," which includes the term "Papovavirus." Instead, this former family name, "Papovaviridae," has now been split into two distinct and separate families, "Papillomaviridae" and "Polyomaviridae." As such, all cultures containing HPV should be noted to contain "Papillomavirus" (signifying its origin from the "Papillomaviridae" family) instead of the former "Papovavirus." |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 8,12 D16S539: 11,12 D5S818: 13 D7S820: 8,11 THO1: 7 TPOX: 8 vWA: 17 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | RA Pattillo |
| Deposited As： | Homo sapiens |
| References： | Pattillo RA, et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. PubMed: 867042 Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 |
