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微信小章| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
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| 商品货号： | TS200247 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | NRC 5140 ATCC 66527, C468, CMCC 1398, LL20, NCYC 1445 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | MATalpha leu2-3 leu2-112 his3-11 his3-15 psi+ cir+ |
| Preceptrol®： | no |
| Mating Type： | alpha |
| Ploidy： | haploid |
| Comments： | Expression of Aspergillus awamori glucoamylase gene Sensitive to Kluyveromyces lactis toxin |
| Medium： | ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25.0°C |
| Name of Depositor： | RK Latta |
| References： | Suominen I, et al. Enhanced recovery and purification of Aspergillus glucoamylase from Saccharomyces cerevisiae by the addition of poly(aspartic acid) tails. Enzyme Microb. Technol. 15: 593-600, 1993. PubMed: 7763957 Flory N, et al. Thermosensitive mutants of Aspergillus awamori glucoamylase by random mutagenesis: inactivation kinetics and structural interpretation. Protein Eng. 7: 1005-1012, 1994. PubMed: 7809026 Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863 Erratt JA, Nasim A. Cloning and expression of a Saccharomyces diastaticus glucoamylase gene in Saccharomyces cerevisiae and Schizosaccharomyuces pombe. J. Bacteriol. 166: 484-490, 1986. PubMed: 3009402 McNeil JB, et al. High frequency recombination and the expression of genes cloned on chimeric yeast plasmids: identification of a fragment of 2-um circle essential for transformation. Curr. Genet. 2: 17-25, 1980. Fierobe HP, et al. Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering. Biochemistry 35: 8696-8704, 1996. PubMed: 8679632 |