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| 产品名称： | M3499 URA3 --> ADE2 converter |
|---|---|
| 商品货号： | TS200274 |
| Designations： | M3499 URA3 --> ADE2 converter |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.4260001182556150 Vector: M3499 (plasmid) Construction: pUC18 Marker(s):ampR ADE2 Construct size (kb): 7.426000118255615 Features: gene disruption cassette: ura3::ADE2 marker(s): ampR replicon: pMB1 restriction site: HindIII restriction site: HindIII...EcoRI restriction site: PstI |
| Applications： | YI-type (integrating) shuttle vector host modification marker swap vector URA3 --> ADE2 |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.5, 1.3, 1.1, 1.1, 1.0; BamHI--6.6, 0.5; PvuI--6.2, 0.8. To convert the host phenotype from URA3 to ADE2, transform with the PstI digested vector (4.45 kb) and select for Ade+ transformants. A marker swap (URA3->ADE2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Vector was constructed by replacing an internal StuI-EcoRV fragment of URA3 with a 3.7 kb BamHI fragment containing the ADE2 coding sequence. ADE2 and URA3 are in the same orientation. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Kasten MM, et al. A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators.. Mol. Cell. Biol. 17(8): 4852-4858, 1997. PubMed: 9234741 |
| Related Products： | component of:ATCC 87561 |
| Shipped： | frozen |