微信小陈
微信小章| 产品名称： | JVM-13 |
|---|---|
| 商品货号： | TS200276 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells infected with Epstein-Barr virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | B-prolymphocytic leukemia (B-PLL) |
| Age： | late 60s |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Modal chromosome number: 47This is a pseudodiploid human cell line of male origin with a modal chromosome number of 47. Most of the cells contain the derivative chromosomes: add(2q), t(8;13)(p12;q13) and t(8;?)(p12;?). Overall, the karyology was consistent with other published data PubMed: 3262465. |
| Images： |  |
| Derivation： | Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. |
| Clinical Data： | Late 60s
Caucasian
male |
| Antigen Expression： | CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC) |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 104xa0to 4 x 104xa0viable cells/mL. Do not allow the cell concentration to exceed 1 x 106xa0cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
|
| Cryopreservation： | 70% growth medium; 20% FBS; 10% DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,12 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 9 THO1: 6,7 TPOX: 10,11 vWA: 16,18 |
| Population Doubling Time： | about 58 hours |
| Name of Depositor： | J.V.Melo |
| References： | Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393 Melo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465 Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118. Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |