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| 产品名称： | pMB |
|---|---|
| 商品货号： | TS200295 |
| Designations： | pMB |
| Depositors： | JJ McCormick |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5999999046325680 Vector: pMB (plasmid) Promoters: Promoter MMTV LTR Construction: pM (ATCC 77383) Marker(s):ampR Construct size (kb): 5.599999904632568 Features: insert detection: lacZ insert detection: lacZamb5 marker(s): ampR promoter: MMTV LTR replicon: pMB1 MCS: EcoRI...HindIII MCS: NruI...HindIII restriction site: ClaI restriction site: NotI restriction site: NsiI terminator: SV40 enhancer: RSV |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--2.5, 1.8, 1.3; EcoRI--3.0, 2.6; HindIII--2.6, 1.7, 1.3; BglII--5.6; ClaI--4.3, 1.3. The plasmid contains the following restriction sites (bp from position 0); NotI--0; EcoRI--9, 3071; ClaI--425, 1560, 1745; Drd--1398, 3583, 5452; PvuI--1596, 2953, 4741; BglII--1782; SphI--2907, 3110; NsiI--2909; HindIII--99, 1794, 3112; BsaI--4441. Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by an RSV enhancer-MMTVLTR promoter at one end and splicing and polyadenylation signals at the other. The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962 J J McCormick, personal communication |
| Related Products： | component of:ATCC 87057 |
| Shipped： | freeze-dried |