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| 产品名称： | c9315 |
|---|---|
| 商品货号： | TS200310 |
| Designations： | c9315 |
| GenBank Number： | U10397 U10398 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 35.40000152587891 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: FUR1 Gene name: uracil phosphoribosyltransferase Contains complete coding sequence?: Y Chromosome: VIII Genome: Saccharomyces cerevisiae Gene symbol: ACT5 Gene name: centractin Contains complete coding sequence?: Y Chromosome: VIII Genome: Saccharomyces cerevisiae Gene symbol: YCK1 Gene name: casein kinase 1 homolog Contains complete coding sequence?: Y Chromosome: VIII Genome: Saccharomyces cerevisiae Gene symbol: SPS100 Gene name: sporulation-specific wall maturation protein Contains complete coding sequence?: Y Chromosome: VIII Genome: Saccharomyces cerevisiae Gene symbol: RPL4B Other gene symbols: MAK18 Gene name: ribosomal protein L41 Contains complete coding sequence?: Y Chromosome: VIII Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 28.995 Cross references: DNA Seq. Acc.: U10397 DNA Seq. Acc.: U10398 Insert lengths(kb): 28.9950008392334 Gene product: uracil phosphoribosyltransferase ACT5 |
| Insert Size (kb)： | 28.995 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen, uncharacterized |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 4.8, 4.3, 3.4, 2.9 (doublet), 2.6, 2.5 and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.579 (vector), 4.723, 4.194, 3.339, 2.994, 2.871, 2.652, 2.478, 1.668, 1.465, 1.338, 1.334, 1.162 (vector), 0.998, 0.871, 0.788, 0.713, 0.475, 0.445, 0.407. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 357767-386762 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |