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| 产品名称： | pSP64-Aa198 |
|---|---|
| 商品货号： | TS200321 |
| Designations： | pSP64-Aa198 |
| Species： | Haemophilus actinomycetemcomitans (Klinger) Potts et al. |
| Depositors： | OmniGene, Inc., MC Chen, OmniGene, Inc. |
| Applications： | This probe can be used to detect Actinobacillus actinomycetemcomitans (proposed to be reclassified as Haemophilus actinomycetemcomitans), a causative agent of juvenile periodontitis. |
| Vector： | Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSP64 Intact vector size: 2.999 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: SP6, -> MCS: HindIII...EcoRI, -> enhancer: none replicon: pMB1 marker(s): ampR terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Haemophilus actinomycetemcomitans Gene name: genus specific DNA Contains complete coding sequence?: U Strain: Y4 Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 1.2 Cross references: Insert lengths(kb): 1.200000047683716 Gene product: genus specific DNA |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; HindIII--4.2; EcoRI--4.2; Pvul--4.2; PstI--4.2; EcoRI/PstI--3.2, 0.9. This probe can be used to detect Actinobacillus actinomycetemcomitans (proposed to be reclassified as Haemophilus actinomycetemcomitans), a causative agent of juvenile periodontitis. This probe does not hybridize to Haemophilus aphrophilus (strains 621, 626, 654, 655, H77, H80, H81, ATCC 13252, ATCC 19415, NCTC 5906), H. paraprophilus (strains H76, H78, ATCC 29241), or H. influenza (strains H9, H104). This probe hybridizes to A. actinomycetemcomitans, strains Y4, 511, 652, ATCC 29522, ATCC 29523, ATCC 29524, 650, 651, 652, 2043, 2097, 2112, N27, NCTC 9710. |
| References： | Chen MC, et al. Detection of human oral cells by nucleic acid hybridization. US Patent 4,866,167 dated Sep 12 1989 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |