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| 产品名称： | p416 GPD |
|---|---|
| 商品货号： | TS200341 |
| Designations： | p416 GPD |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.8039999008178710 DESCRIPTION OF VECTOR: Intact vector size: 5.804 Type of vector: plasmid Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI Other unique sites: SacI Construction: pRS416 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): replicon: CEN6/ARSH4, -> marker(s): URA3, -> terminator: CYC1, -> promoter for expression: GPD, marker(s): ampR, Vector: p416 GPD (plasmid) Promoters: Promoter for expression GPD Construction: pRS416 Marker(s):URA3,ampR Construct size (kb): 5.803999900817871 Features: marker(s): URA3 marker(s): ampR promoter for expression: GPD replicon: CEN6/ARSH4 terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): SstI/XbaI--5.13, 0.68; EcoRI--5.8, BamHI--5.8. Low copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |