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| 产品名称： | pSP64-VIII |
|---|---|
| 商品货号： | TS200459 |
| Designations： | pSP64-VIII |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc., JJ Toole, Genetics Institute, Inc. |
| Vector： | Construct size (kb): 10.19999980926514 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSP64 Intact vector size: 2.999 Type of vector: plasmid Vector end: SalI Vector end: SalI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: SP6, -> MCS: HindIII...EcoRI, -> enhancer: none replicon: pMB1 marker(s): ampR terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: F8C Genomic copy number: unique Gene name: coagulation factor VIIIc, procoagulant component (hemophilia A) Contains complete coding sequence?: U Chromosome: X; Localization: X q28 Tissue: fetal liver Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 7.2 Cross references: Insert lengths(kb): 7.199999809265137 Tissue: fetal liver Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) F8C Alleles: F3, E1, E1, E2, F1, F2, A1, A2 |
| Insert Size (kb)： | 7.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | PvuI and SmaI are unique restriction sites. Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.0, 2.6; EcoRI--4.9, 3.4, 1.8, 0.4. The insert contains cDNA sequences from just before the initiator ATG (nucleotide 150-152) to nucleotide 7422 (200 bp downstream from the terminator at nucleotide 7201). The insert detects a single mRNA of about 9 kb from human liver. This was constructed by ligating several independently isolated cDNA clones and adding a SalI linker at nucleotide 145. |
| References： | Toole JJ Jr.. Novel procoagulant proteins. US Patent 4,868,112 dated Sep 19 1989 Toole JJ, et al. Molecular cloning of a cDNA encoding human antihaemophilic factor. Nature 312: 342-347, 1984. PubMed: 6438528 John J Toole, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |