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| 产品名称： | pG104/T1 |
|---|---|
| 商品货号： | TS200562 |
| Designations： | pG104/T1 |
| GenBank Number： | M34531 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff, B Repetto |
| Vector： | Construct size (kb): 13.69999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: dihydrolipoyl transsuccinylase component of the alpha-ketoglutarate dehydrogenase complex KGD2 |
| Insert Size (kb)： | 6.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (in approximate kb from the end of the insert closer to the 5 end of the gene): SstI--0.2, EcoRI--0.3, HindIII--1.0, 4.3; BamHI--1.05; PstI--1.2; BalI--1.4, 1.9, BglII--4.7, 5.3. Restriction digests of the clone give the following sizes (kb): BamHI--13.6; EcoRI--9.4, 2.3, 1.9; HindIII--5.4, 3.8, 2.1, 1.2, 1.1. Upstream flanking regions, starting from the HaeIII site at -322, contain the regulatory elements necessary for glucose-repressible transcription. HAP-regulated transcription requires sequences between a Sau3A site at -484 and the HaeIII (-322) site. Both a 675 bp BclI fragment from within the reading frame and a BamHI/PstI fragment from the upstream region detect 2 transcripts of 2.3 kb and 2.0 kb in approximately equal abundance. There are 2 start sites for transcription, -109 and -254. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Repetto B, Tzagoloff A. Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase. Mol. Cell. Biol. 10: 4221-4232, 1990. PubMed: 2115121 |