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| 产品名称： | pMS2 |
|---|---|
| 商品货号： | TS200599 |
| Designations： | pMS2 |
| Depositors： | G Strachan, Allelix Biopharmaceuticals, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Escherichia coli RR1 |
| Vector Information： | Construct size (kb): 21.00
Vector: pMS2 (plasmid)
Construction: pBR322; pMS1
Marker(s): ampR, kanR
Replicon: pMB1, pMS1
Cloning sites: BamHI, EcoRI
Possible host(s): E. coli, Corynebacteriumxa0(ATCC® 13059™), Rhodococcus equi |
| Applications： | shuttle vector |
| Comments： | Ampicillin and kanamycin resistance are expressed in E. coli; kanamycin resistance is expressed in Rhodococcus equi.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990Restriction digests of the clone give the following sizes (kb): BamHI--21.0; EcoRI--21.0; HindIII--6.8 (doublet), 3.6, 1.45, 1.1.
-ATCC staffpBR322 cut with BamHI, and pMS1 (a 19 kb plasmid from Rhodococcus equi) partially digested with Sau3AI, were ligated to form pMS2. The construct retains ampR and ori from pBR322 and kanR and ori from pMS1.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990A shuttle vector stably maintained in E. coli as well as members of the coryneform group, namely Corynebacterium (ATCC® 13059™) and Rhodococcus equi.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Kozlowski M, Glasse-Davies W. Shuttle vectors from Rhodococcus equi. US Patent 4,920,054 dated Apr 24 1990 |
| Shipped： | frozen |
| Shipping Information： | Escherichia coli RR1 containing plasmid |