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| 产品名称： | ptac12 |
|---|---|
| 商品货号： | TS200634 |
| Designations： | ptac12 |
| Depositors： | E Amann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.60 Vector: ptac12 (plasmid) Promoters: Promoter tac Construction: pEA300 (ATCC 37181), pGL101 Marker(s):ampR Construct size (kb): 2.6 Features: marker(s): ampR promoter: tac replicon: pMB1 enhancer: none |
| Applications： | contains easily purifiable cassette(s) for construction expression vector |
| Comments： | A 55 bp HpaII/PvuII fragment containing the -10 region of the lacUV5 promoter was inserted into the ClaI site of pEA300 (ATCC 37181). Contains the lac operator. The PvuII site is located 5 bp downstream of the lacZ ribosome binding sequence. The tac promoter can be excised by a EcoRI/PvuII digest. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1982. Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid. |