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| 产品名称： | p424 ADH |
|---|---|
| 商品货号： | TS200722 |
| Designations： | p424 ADH |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.3470001220703130 Vector: p424 ADH (plasmid) Promoters: Promoter for expression ADH Construction: pRS424 Marker(s):TRP1,ampR Construct size (kb): 7.347000122070313 Features: marker(s): TRP1 marker(s): ampR promoter for expression: ADH replicon: 2 micron MCS: XhoI...XbaI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.4; EcoRI--7.4; SacI/XbaI--3.2, 1.7, 1.5, 1.0. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | freeze-dried |