| 产品名称: | P1 F9, F9 P1, F9.P1, FIX, FIX P1 |
|---|---|
| 商品货号: | TS200747 |
| Designations: | P1 F9, F9 P1, F9.P1, FIX, FIX P1 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 9.399999618530273 |
| Insert: | DNA: genomic Insert lengths(kb): 5.0 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) F9 Alleles: A1, A2, D2, C1, D1, C2 |
| Insert Size (kb): | 5.0 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--5.21, 4.83; HindIII--7.99, 2.53; BamHI--12.41; PstI--12.11; PvuII--7.99, 3.67. The insert can be separated after a EcoRI/HhaI digest of the clone. The insert is not cut but the vector is digested to fragments <400 bp. The XmnI RFLP has demonstrated linkage to X-linked albinism-deafness syndrome (ADFN) at about 8.5 cM. This probe does not detect the MspI RFLP. The depositor recommends hybridization at 42C in 40% formamide and washing at 60C, 0.5X SSC, 0.1% SDS. |
| References: | Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147 Shiloh Y, et al. Genetic mapping of X-linked albinism-deafness syndrome (ADFN) to Xq26.3-q27.1. Am. J. Hum. Genet. 47: 20-27, 1990. PubMed: 2349949 Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485 Jean Louis Mandel, personal communication |