宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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P1 [F9, F9 P1, F9.P1, FIX, FIX P1]

货号 TS200747
中文名称 null
适应种属
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产品名称: P1 F9, F9 P1, F9.P1, FIX, FIX P1
商品货号: TS200747
Designations: P1 F9, F9 P1, F9.P1, FIX, FIX P1
Species: Homo sapiens, human
Vector:
Construct size (kb): 9.399999618530273
Insert:
DNA: genomic
Insert lengths(kb): 5.0
Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) F9
Alleles: A1, A2, D2, C1, D1, C2
Insert Size (kb): 5.0
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.21, 4.83; HindIII--7.99, 2.53; BamHI--12.41; PstI--12.11; PvuII--7.99, 3.67.
The insert can be separated after a EcoRI/HhaI digest of the clone. The insert is not cut but the vector is digested to fragments <400 bp.
The XmnI RFLP has demonstrated linkage to X-linked albinism-deafness syndrome (ADFN) at about 8.5 cM.
This probe does not detect the MspI RFLP.
The depositor recommends hybridization at 42C in 40% formamide and washing at 60C, 0.5X SSC, 0.1% SDS.
References:

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Shiloh Y, et al. Genetic mapping of X-linked albinism-deafness syndrome (ADFN) to Xq26.3-q27.1. Am. J. Hum. Genet. 47: 20-27, 1990. PubMed: 2349949

Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485

Jean Louis Mandel, personal communication