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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS200792 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/Virginia/ATCC2/2009 |
| Applications： | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin. |
| Effect on Host： | CPE, rounded refractile cells with cell degeneration |
| Recommended Host： | MDCK (ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-5 days
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| Name of Depositor： | ATCC |
| Special Collection： | ATCC |
| Source： | Nasopharyngeal specimen from a clinical patient, 2009 |