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| 产品名称： | Encephalomyocarditis virus (Murine) (tissue culture adapted) |
|---|---|
| 商品货号： | TS200894 |
| Classification： | Picornavirus, Cardiovirus |
| Deposited As： | Encephalomyocarditis (Murine) (tissue culture adapted) |
| Agent： | Encephalomyocarditis virus (Murine) (tissue culture adapted) |
| Strain： | EMC |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This strain is indistinguishable antigenically from strain MM and Mengo encephalomyocarditis virus. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | Vero (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.xa0 End adsorption by adding virus growth medium.
Incubation: 2-4 days |
| Name of Depositor： | W Brandt |
| Source： | Chimpanzee, Florida, 1944 |
| Year of Origin： | 1944 |
| References： | Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945. |