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| 产品名称： | pMWNT3 plasmid in E. coli GC10 |
|---|---|
| 商品货号： | TS201187 |
| Designations： | pMWNT3 plasmid in E. coli GC10 |
| GenBank Definition： | Mutated version- (tryptophan 539 to leucine) |
| GenBank Number： | M32502 |
| Species： | Mus musculus, mouse |
| Depositors： | Roel Nusse |
| Vector： | Construct size (kb): 4.6 |
| Insert： | DNA: cDNA Gene: Wingless-type MMTV integration site family member 3, Wnt-3 Source: mouse Insert size (kb): 1.4 Excise insert: EcoRI |
| Putative ID： | wingless-related MMTV integration site 3 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
| Comments： | This is a clone of the mouse Wnt-3 cloned into the EcoRI site of the pGEM-3Zf vector. The insert corresponds to approximately nt 170-1570 of the Wnt-3 sequence in Genbank (M32502). Restriction digests of this clone give the following sizes (in kb): SmaI - 4.4, 0.45; EcoRI - 3.5, 1.5. - ATCC staff |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993. |