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| 产品名称： | pGC25 |
|---|---|
| 商品货号： | TS201197 |
| Designations： | pGC25 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7839999198913570 Vector: pGC25 (phagemid) Promoters: Promoter for in vitro transcription T3 Construction: pRS402 pJK142 pRS411 Marker(s):ampR Construct size (kb): 3.783999919891357 Features: marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: SacI/.../KpnI centromere: CEN6 coding sequence: lacZ |
| Applications： | YC-type (centromeric) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.8; EcoRI--3.8; XbaI--3.8. This vector lacks a selectable marker in yeast. It will contribute many useful features as a base vector when used to construct new vectors that possess yeast selectable markers. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Shipped： | freeze-dried |