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| 产品名称： | RATREN |
|---|---|
| 商品货号： | TS201264 |
| Designations： | RATREN |
| GenBank Number： | J02941 |
| Species： | Rattus norvegicus, rat |
| Depositors： | KR Lynch |
| Vector： | Construct size (kb): 4.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4Z Intact vector size: 3.000 Type of vector: plasmid Vector end: BamHI Vector end: HindIII Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene symbol: REN Genomic copy number: unique Gene name: renin Contains complete coding sequence?: Y Strain: Sprague-Dawley Tissue: kidney, male Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 1.4 Cross references: DNA Seq. Acc.: J02941 Nucleotides 1-1432 of the insert correspond to nucleotides 184-1615 of J02941. Insert lengths(kb): 1.399999976158142 Tissue: kidney, male Gene product: renin REN Alleles: A1, A2 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.0, 1.4; PstI--3.4, 0.95; BamHI--4.3. Constructed by isolating a 1.4 kb EcoRI fragment from a partial digest of a clone in lambdagt10 and cloning the fragment into a pBluescript vector. A 1.4 kb BamHI/HindIII fragment was excised and cloned into pGEM-4Z. |
| References： | Burnham CE, et al. Molecular cloning of rat renin cDNA and its gene. Proc. Natl. Acad. Sci. USA 84: 5605-5609, 1987. PubMed: 3039496 Kevin R Lynch, personal communication |