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微信小章| 产品名称： | UMB1949 |
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| 商品货号： | TS201403 |
| Organism： | Homo sapiens, human |
| Tissue： | Kidney; angiomyolipoma |
| Cell Type： | Melanocyte immortalized with hTERT and SV40 large T antigen |
| Product Format： | frozen |
| Morphology： | Epithelial-like |
| Culture Properties： | Adherent |
| Biosafety Level： | 2 xa0Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Tuberous sclerosis |
| Age： | 36 years |
| Gender： | Male |
| Applications： | This cell line can be used to study signal transduction and drug efficiency in tuberous sclerosis. |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Karyotype： | This cell line is of male origin and 1/2 to 2/3 of the total cell population is pseudodiploid, the rest of the cells fall in the tetraploid range. Consistent cytogenetic changes include chromosome 10 and 19 aberration, and chromosome 4 monosomy. Some cells showed loss of the Y chromosome and many of the examined cells contained random chromosomal aberrations. UMB1949 cells have a defined 5bp deletion in exon 33 of tuberin (Tsc2) and mutations in tuberin (and/or hamartin) cause tuberous sclerosis. |
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| Derivation： | The UMB1949 cell line was established by sequential introduction of the SV40 large T antigen and human telomerase into human angiomyolipoma cells.xa0 |
| Antigen Expression： | Antigen expression: positive for epithelial marker pan-cytokeratin (immunocytochemistry) |
| Comments： | Angiomyolipomas are benign tumors of the kidney which originate from putative perivascular epithelioid cells that may undergo differentiation into cells with features of melanocytes, smooth muscle or fat cells.xa0
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended.
Medium renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Aminal Cells: A Manual of Basic Techniques by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: XY CSF1PO: 10, 11 D13S317: 12, 13 D16S539: 12 D5S818: 11 D7S820: 9, 10 THO1: 6, 8 TPOX: 12 vWA: 18 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | JL Arbiser |
| Year of Origin： | June 2004 |
| References： | Arbiser JL, et al. The generation and characterization of a cell line derived from a sporadic renal angiomyolipoma: use of telomerase to obtain stable populations of cells from benign neoplasms. Am. J. Pathol. 159: 483-491, 2001. PubMed: 11485907 Lim SD , et al. Expression of the neural stem cell markers NG2 and L1 in human angiomyolipoma: are angiomyolipomas neoplasms of stem cells? Mol. Med. 13(3-4): 160-165, 2007. PubMed: 17592550 Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13. |