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| 产品名称： | pTrcrham-Iso2 Rhamiso 2 |
|---|---|
| 商品货号： | TS201422 |
| Designations： | pTrcrham-Iso2 Rhamiso 2 |
| GenBank Number： | X60472 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | C Wong |
| Applications： | produces protein L-rhamnose isomerase |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.299999952316284 Gene product: L-rhamnose isomerase rhaA Target Gene: L-rhamnose isomerase |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI/HindIII--4.3, 1.3; EcoRI--uncut; BamHI--5.0, 0.62. Constructed by amplification from genomic DNA using the primers: 5-ATATTCTGCAGACCACTCA-3, generating a PstI site within the natural AUG codon, and 5-GGCGCAAGCTTTTACCTGCTGGCGTATCGTTGT-3, generating a HindIII site after the stop codon. Initiation and termination sequences were then ligated to the respective ends. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Chi-Huey Wong, personal communication |